Phosphor image analysis of radioactivity in the retina of rabbits after a single intravitreal injection of GEM132
| Days Postdose | Dose (μg/eye) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 3.7 | 15.7 | 78.5 | |||||||
| A | B | C | A | B | C | A | B | C | |
| Percentage of total sample radioactivity in gel regions4-a (mean, S.D.) | |||||||||
| 7 | ND | ND | ND | ND | ND | ND | 100 (0) | 0 | 0 |
| 14 | 1004-b | 0 | 0 | 100 (0) | 0 | 0 | 94.7 (4.7) | 5.34-b | 0 |
| 28 | 87.64-b | 12.54-b | 0 | 90.7 (2.1) | 9.3 (2.1) | 0 | 86.1 (0.7) | 13.9 (0.7) | 0 |
| 56 | 79.0 (10.6) | 20.1 (10.6) | 0 | 69.4 (3.9) | 19.8 (9.9) | 10.8 (9.8) | 71.6 (8.9) | 15.5 (3.8) | 13.0 (5.2) |
| 114/1494-c | 51.1 (1.5) | 48.9 (1.5) | 0 | 46.1 (2.6) | 42.3 (11.9) | 11.6 (10.7) | 45.1 (10.4) | 35.1 (2.8) | 19.8 (7.6) |
ND, not determined.
↵4-a Region A, radioactivity with the same mobility as GEM132 and n-1 + n-2 impurities/metabolites. Region B, radioactivity of impurities/metabolites of chain lengthn ≥ 3. Region C, radioactivity of molecular species with higher molecular mass than GEM132. Values reflect the percentage of the radioactivity in the extracted samples migrating in each region.
↵4-b Mean, n = 2.
↵4-c For the low- and mid-dose groups, the last time point was 149 days postdose. For the high-dose group, the last time point was 114 days postdose.