Table 1

Glucuronidation of 4-NP and NNAL in human liver and lung microsomes

Microsome Specimen4-NP-Gluc Formation1-aNNAL-Gluc Formation1-bNNAL-Gluc II:NNAL- Gluc I1-c
μmol/mg nmol/mg
Liver no. 22ND1-d 2.63.5
Liver no. 109ND0.62.8
Liver no. 612973.11.03.8
Lung no. 176820.015Not detected
Lung no. 177020.024Not detected
Lung no. SEM0.004Not detected
Lung no. 996040.006Not detected
RLM (PB1)1-e 2.80.70.2
  • 1-a  4-NP glucuronidation assays were performed for 2 h using microsomal protein (50 μg) from rat liver, human liver, and human lung tissues.

  • 1-b  NNAL glucuronidation assays were performed for 2 h using microsomal protein from rat liver (50 μg, 40 min incubation), human liver (0.1 mg, 40 min incubation), and human lung (0.1–2.5 mg, 2 h incubation) tissues.

  • 1-c  Ratios of NNAL-Gluc II to NNAL-Gluc I in glucuronidation assays using microsomal protein from rat liver, human liver, and human lung tissues.

  • 1-d  ND, not done.

  • 1-e  RLM, rat liver microsomes from a phenobarbitol-treated rat, PB1 (see Ren et al., 1999).