Formation of 4′-HPPH, 3′-HPPH, 3′,4′-diHPPH, and 3′,4′-dihydrodiol in reconstituted systems containing purified recombinant P450 enzymes
| P450 | Substrate (μM) | ||||||
|---|---|---|---|---|---|---|---|
| DPH (100 μM) | 4′-HPPH (1 μM) | 4′-HPPH (10 μM) | 4′-HPPH (100 μM) | ||||
| 4′-HPPH | 3′-HPPH | 3′,4′-diHPPH | 3′,4′-dihydrodiol | 3′,4′-diHPPH | 3′,4′-diHPPH | 3′,4′-diHPPH | |
| pmol product/min/nmol P450 | |||||||
| CYP1A2 | 2 | 2 | 2 | 23 | <0.1 | <0.1 | 13 |
| CYP2C9 | 226 | 3 | <0.1 | 2 | 2 | 4 | 41 |
| CYP2C19 | 154 | 9 | 10 | 10 | 16 | 105 | 733 |
| CYP3A4 | <0.1 | <0.1 | <0.1 | 2 | 1 | 4 | 30 |
DPH (100 μM) or 4′-HPPH (1, 10, and 100 μM) was incubated at 37°C for 30 min in reconstituted systems (0.20 μM P450) as described underMaterials and Methods. Results are presented as means of duplicate determinations.