Compound | Retention Time | Concentration | |||
---|---|---|---|---|---|
Nontreated Plasma | Treated Plasma4-a | ||||
Sample 2 h | Sample 4 h | Sample 2 h | Sample 4 h | ||
min | μM | ||||
7-Glucuronide (3) | 7.95 | N.D. | 0.23 ± 0.02 | N.D. | N.D. |
20-Glucuronide (2) | 13.10 | 0.14 ± 0.04 | 0.36 ± 0.04 | N.D. | N.D. |
Silybin A (1) | 17.44 | 0.15 ± 0.01 | 0.61 ± 0.04 | 0.30 ± 0.01 | 0.95 ± 0.03 |
Silybin B (1) | 17.80 | 0.06 ± 0.00 | 0.23 ± 0.02 | 0.25 ± 0.02 | 1.19 ± 0.07 |
Results are expressed as means ± S.D. from three analyses.
N.D., not determined.
↵4-a Plasma was treated for determination of total amount of silybin (conjugated and unconjugated) with β-glucuronidase/arylsulfatase mixture for 4 h at 37°C in shaking water bath for enzymatic cleavage of silybin conjugates (Weyhenmeyer et al., 1992; Mascher et al., 1993; Rickling et al., 1995).