Sample | RAF | Clearance | Contribution | Inhibition by Anti-CYP1A2 Antibody3-a | ||||
---|---|---|---|---|---|---|---|---|
CYP1A2 | CYP3A4 | CYP1A2 | CYP3A4 | HL3-b | CYP1A2 | CYP3A4 | ||
nmol CYP/mg | μl/mg/min | % | % | |||||
HL114 | 0.160 | 0.133 | 1.96 | 0.29 | 4.49 | 43.7 | 6.5 | 48 |
HL212 | 0.264 | 0.150 | 3.23 | 0.33 | 5.30 | 60.9 | 6.2 | 50 |
HL214 | 1.074 | 0.272 | 13.13 | 0.60 | 17.21 | 76.3 | 3.5 | 60 |
HL215 | 0.092 | 0.118 | 1.13 | 0.26 | 6.10 | 18.3 | 4.2 | 24 |
RAFs and the contributions of CYP1A2 and CYP3A4 were calculated as described in Experimental Procedures. The clearances of pimobendan O-demethylation by recombinant CYP1A2 and CYP3A4 were 12.23 and 2.19 μl/nmol of CYP/min, respectively.