Treatment | Fold CAT Activity versus Control | |
---|---|---|
pTX1X1/inv | pBLh1A1CAT | |
Control | 1 (arb) | 1 (arb) |
MET | 1.2 ± 0.2 | 1.2 ± 0.13 |
DEX | 1.0 ± 0.1 | 1.0 ± 0.13 |
MET + DEX | 1.5 ± 0.2* | 2.1 ± 0.331-a |
TCDD | 8.6 ± 0.5* | 4.9 ± 0.471-a |
TCDD + DEX | 8.0 ± 0.5* | 7.5 ± 1.01-a |
HepG2 cells were transfected with either pTXIX/inv or pBLh1A1CAT and treated with inducers as outlined in Materials and Methods. Control, vehicle solvent (0.1% v/v ethanol); MET, 500 μM metyrapone; DEX, 100 nM dexamethasone; MET + DEX, 500 μM metyrapone and 100 nM dexamethasone; 1 nM TCDD; TCDD + DEX, 1 nM TCDD and 100 nM dexamethasone. Data are expressed as the mean ± S.D. of fold activity versus control of three determinations from the same experiment, typical of two separate experiments.
↵1-a Statistically different-fold induction versus control using Student's t test (two-tailed), P > 95%.