Identification of quinols formed by recombinant CYP1A1
| Retention Time | Peak Ratio | |
|---|---|---|
| min | ||
| 10β-Hydroxy-1,4-estradiene-3,17-dione | (286.3/268.3/145.0/124.0/123.0) | |
| Authentic compound | 8.22 | 1/0.49/3.4/3.1/2.8 |
| Product | 8.19 | 1/0.51/3.7/3.2/2.9 |
| 10β,17β-Dihydroxy-1,4-estradiene-3-one | (288.3/270.3/147.0/124.0/123.0) | |
| Authentic compound | 8.45 | 1/0.58/8.1/6.3/5.6 |
| Product | 8.39 | 1/0.62/8.4/6.5/5.9 |
Reaction conditions are described in Experimental Procedures.