Electrospray mass spectra of biliary metabolites of [14C]AM
| Metabolite | Spectrum |
|---|---|
| I (dihydroxy AM.Gluc) | m/z 524 ([M+NH4]+, 100), 492 ([M+NH4−CH3OH]+, 21), 267 ([492−NH3−DHG−O2]+, 96) |
| II (hydroxy AM.Gluc) | m/z 508 ([M+NH4]+, 6), 476 ([M+NH4−CH3OH]+, 54), 459 ([476−NH3]+, 100), 283 ([459−DHG]+, 10), 265 ([283−H2O]+, 5), 237 ([265−CO]+, 2) |
| III (9α-hydroxy AM.Gluc) | m/z 508 ([M+NH4]+, 98), 476 (59), 459 (57), 283 (15), 265 (100), 247 ([265−H2O]+, 28), 237 (44), 219 ([237−H2O]+, 9), 205 (9), 179 (14) |
| IV (hydroxy AM.Gluc) | m/z 508 ([M+NH4]+, 85), 476 (45), 459 (100), 283 (10), 265 (29), 247 (40), 237 (73), 219 (29), 205 (4), 179 (29), 161 (21) |
| Furano acetate Gluc2-a | m/z 478 ([M+NH4]+, 100), 401 ([M+NH4−NH3−CH3O2H]+, 16), 373 ([401−CO]+, 22), 267 ([M+NH4−NH3−DHG−H2O]+, 27), 225 ([401−DHG]+, 38), 207 ([225−H2O]+, 51) |
| V (hydroxy AM.Gluc) | m/z 508 ([M+NH4]+, 84), 476 (3), 459 (8), 283 (2), 265 (11), 251 ([283−O2]+, 100), 237 (13), 219 (73) |
| VI (hydroxy AM.Gluc) | m/z 508 ([M+NH4]+, 100), 476 (16), 459 (21), 283 (3), 265 (40), 247 (6), 237 (64) |
| VII (DHA.Gluc) | m/z 478 ([M+NH4]+, 100), 267 ([M+NH4−NH3−DHG−H2O]+, 53), 221 ([267−H2O−CO]+, 14), 163 ([221−(CH3)2CO)]+, 30) |
Metabolites were resolved by HPLC. Mass spectra acquired at a cone voltage of 70 V. Roman numerals refer to peaks in radiochromatogram (Fig. 2).
↵2-a Metabolite not seen consistently as a radiolabeled peak.