Table 2

Examination of coupled oxidation and glucuronidation of 7-ethoxycoumarin by human liver microsomes

Addition	7-Hydroxycoumarin^2-a	7-Hydroxycoumarin Glucuronide^2-a
+NADPH	582 ± 2	ND^2-b
+NADPH + UDPGA	261 ± 20	292 ± 5
+NADPH + alamethicin	608 ± 11	ND^2-b
+NADPH + UDPGA + alamethicin	100 ± 1	502 ± 4

7-Ethoxycoumarin (250 μM) and NADPH were incubated with microsomes from a mixture of nine human livers in the presence and absence of alamethicin and/or UDPGA. Incubations were then analyzed as described in Materials and Methods, and data is presented as the mean ± S.D. of three determinations.

↵2-a Picomoles per milligram of protein per minute.
↵2-b ND = not detected.