Substrate | Microsomes | Cytosol | Microsomes + Cytosol | |||
---|---|---|---|---|---|---|
Product | Activity | Product | Activity | Product | Activity | |
nmol/min/mg of protein | ||||||
DPDS | DPDSO | 4.51 ± 0.3 | DPDSO | uld | DPDSO | 0.04 ± 0.0 |
PM | uld4-a | PM | 5.22 ± 1.0 | PM | 10.60 ± 0.2 | |
PGS | uld 4-a | PGS | 5.41 ± 0.1 | PGS | 1.95 ± 0.0 | |
DPDSO | PGS | 76.79 ± 2.9 | ||||
PM | PGS | 1.65 ± 0.3 |
When DPDS was the substrate, DPDSO was formed by microsomal enzymes, and PM and PGS by cytosolic enzymes. When DPDSO or PM were incubated, only PGS was formed by cytosolic enzymes. Results are means of four replicate experiments ± S.E.
↵4-a uld, under limit of detection (<0.001 ± 0.000 mM for DPDSO; 0.05 ± 0.01 mM for PM).