Treatment | Liver1-a | Heart1-b | Rete Mirabile1-c | |||
---|---|---|---|---|---|---|
CYP1A | EROD | CYP1A | EROD | CYP1A | EROD | |
pmol/mg | pmol/min/mg | pmol/mg | pmol/min/mg | pmol/mg | pmol/min/mg1-d | |
Control | 2 ± 4 | 96 ± 55 | ND | 2 ± 2 | ND | 0.7 |
BP (0.1 mg/kg) | 11 ± 5 | 167 ± 29 | 0.06 | 4 ± 2 | ND | 1.4 |
BP (1 mg/kg) | 58 ± 12* | 422 ± 234* | 0.19 ± 0.10 | 4 ± 2 | 0.03 | 3.5 |
BP (10 mg/kg) | 157 ± 37* | 1312 ± 600* | 3.32 ± 3.45 | 13 ± 12 | 0.70 | 6.3 |
BNF (10 mg/kg) | 122 ± 34* | 1115 ± 901* | 0.97 ± 0.97 | 9 ± 6 | 0.37 | 6.3 |
TCB (10 mg/kg) | 66 ± 27* | 502 ± 191* | 0.43 ± 0.28 | 3 ± 3 | 0.12 | 2.8 |
Eel heart and rete mirabile were characterized for their CYP1A content by Western blot analysis and catalytic activity (EROD) as described under Materials and Methods.
↵1-a Data are from Schlezinger and Stegeman (2000) and are shown as picomole values for ease of comparison. * Significantly different from control at P ≤ .05 (Tukey-Kramer).
↵1-b Data were combined from two experiments. Values for CYP1A are means ± S.D. from two to three pools of two to three individual organs. Values for EROD are means ± S.D. from three to eight pools of two to three individual organs.
↵1-c For rete mirabile, values are from pools of three to five individual organs.
↵1-d EROD activity was measured in postmitochondrial supernatant; microsomal protein from all samples was calculated to represent 14% of the postmitochondrial supernatant. Therefore, the EROD activity in the microsomal fraction was estimated. Microsomal yield per gram of tissue was 8.0 ± 2.8 for heart (n = 26) and 3.0 ± 1.7 for rete (n = 26).