Reproducibility of Ct versus input total RNA
| CypIsozyme | Total RNA Input | Threshold Cycle No. (Ct)2-a | ||
|---|---|---|---|---|
| Mean | S.D. | % CV | ||
| ng | ||||
| 3a11 | 1 | 25.5 | 0.12 | 0.47 |
| 100 | 18.8 | 0.15 | 0.80 | |
| 2b10 | 1 | 29.4 | 0.07 | 0.24 |
| 100 | 23.0 | 0.05 | 0.22 | |
| 2d9 | 1 | 32.1 | 0.03 | 0.09 |
| 100 | 25.7 | 0.07 | 0.27 | |
| 2e1 | 1 | 22.9 | 0.04 | 0.16 |
| 100 | 16.1 | 0.10 | 0.63 | |
| 1a2 | 1 | 31.2 | 0.12 | 0.38 |
| 100 | 24.0 | 0.16 | 0.66 | |
↵2-a Data are from four replicate determinations.