Effect of IBN (I) and 2-MIBN (II) on the activities of testosterone hydroxylases in rat liver microsomes
| Metabolites (Hydroxylated Testosterone) | IC50 | |
|---|---|---|
| IBN (I) | 2-MIBN (II) | |
| μM | ||
| 6β-OH (CYP3A) | <0.2 | 20 |
| 7α-OH (CYP2A) | >100 | >100 |
| 16α-OH (CYP2C) | 22 | 55 |
| 16β-OH (CYP2B) | 3 | 4 |
| 2α-OH (CYP2C) | 22 | 50 |
| 2β-OH (CYP3A) | <0.2 | 10 |
I and II (0.1–100 μM) were incubated with rat liver microsomes (0.2 mg/ml) at 37°C for 5 min in the presence of testosterone (20 μM).