Enzyme kinetic parameters for IBN (I) and 2-MIBN (II) metabolism obtained using immature male rat liver microsomes
| Parameters | IBN (I) | 2-MIBN (II) |
|---|---|---|
| K m (μM) | 0.34 | 18.29 |
| V max (nmol/min/mg protein) | 0.055 | 0.782 |
| V max/K m (ml/min/mg protein) | 0.162 | 0.043 |
| V 6-a ([S] = 0.1 μM) | 0.012 | 0.004 |
| V 6-a ([S] = 0.3 μM) | 0.026 | 0.013 |
| V 6-a ([S] = 1.0 μM) | 0.041 | 0.040 |
| V 6-a ([S] = 5.0 μM) | 0.051 | 0.168 |
The parameters are expressed as mean value obtained from two separate experiments that were carried out in duplicate.
↵6-a Calculated velocity (nanomoles per minute per milligram of protein) using the parameters listed in the table.