Estimated kinetic parameters for the formations of NORCIS, 3-F-4-OHCIS, and 4-F-2-OHCIS from cisapride (1–50 μM) in different HLMs
| HLMs | NORCIS | 3-F-4-OHCIS | 4-F-2-OHCIS | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Km | Vmax | Vmax/Km | Km | Vmax | Vmax/Km | Km | Vmax | Vmax/Km | |
| 8 | 11.3 | 52 | 4.6 | 33 | 28.1 | 0.85 | 7.7 | 14.6 | 1.9 |
| 13 | 23.6 | 278 | 11.8 | 29.4 | 40.2 | 1.4 | 33.2 | 15.6 | 0.47 |
| 16A | 23.1 | 210 | 9.1 | 43.7 | 85.7 | 2.0 | 69.5 | 73.7 | 1.1 |
| 16B | 23.6 | 141 | 6.0 | 14.6 | 42.5 | 2.9 | 19.4 | 38.5 | 1.8 |
| 29 | 35.6 | 93 | 2.6 | 40 | 65 | 1.6 | 26.7 | 9.9 | 0.37 |
| Mean | 23.4 | 154.8 | 6.6 | 32.1 | 52.3 | 1.74 | 31.3 | 30.5 | 1.16 |
| ± S.D. | 8.6 | 90.6 | 3.6 | 11.3 | 22.9 | 0.78 | 23.4 | 26.6 | 0.70 |
Km (micromoles),Vmax (picomoles/minute/milligram of protein), and Vmax/Km(microliters/minute/milligram)