Table 1

HPLC/TSP/MS analyses of proposed PAP metabolites

CompoundMolecular WeightHPLC/TSP/MSRT1-a
Molecular IonFragments
m/z min
PAP-Gluc2852861771.77
PAP-Cys(s)1-b 228, 347, 466, 585348, 5862.30
PAP-GSH(s)1-b 414, 719, 1024130, 147, 198, 238, 308 [GSH + H]+ 2.371-c
APAP-Gluc327152, 159, 176, 2852.371-c
PAP-NACys(s)1-b 270, 389, 508271144, 166, 1872.53, 2.79
APAP-GSH456152, 198, 222, 237, 2792.91
APAP-NACys312146, 152, 187, 222, 2403.84
PAP1091105.44
FPAP1371385.84
N-OH-PAP-O-SO3H2052056.63
APAP1511527.09

  • ↵1-a  Retention time in HPLC chromatogram.

  • ↵1-b  PAP-Cys, PAP-GSH, and PAP-NACys conjugates included mono- and multi-substituted isomers.

  • ↵1-c  The compound was not well separated.