2α-OH Testosterone1-a | HC-Gluc1-b | HC-Sulf1-b | K+Content | Histomorphology1-c | |
---|---|---|---|---|---|
pmol/min/slice | μmol/mg protein | ||||
Liver 1 | |||||
Fresh | 248 ± 37 | 1030 ± 96 | 65 ± 8 | 0.74 ± 0.07 | 67 ± 2 |
Cryopreserved | 207 ± 39 | 816 ± 11 | 52 ± 0 | 0.49 ± 0.03 | 53 ± 8 |
% fresh | 84 ± 20 | 79 ± 7 | 80 ± 9 | 67 ± 7 | 79 ± 11 |
Liver 2 | |||||
Fresh | 338 ± 28 | 1298 ± 66 | 63 ± 9 | N.D. | 75 ± 0 |
Cryopreserved | 214 ± 20 | 693 ± 110 | 41 ± 8 | N.D. | 32 ± 2 |
% fresh | 63 ± 8 | 53 ± 9 | 65 ± 16 | N.D. | 42 ± 2 |
Liver 3 | |||||
Fresh | 156 ± 11 | 1301 ± 30 | 64 ± 5 | 0.71 ± 0.03 | 72 ± 2 |
Cryopreserved | 133 ± 16 | 904 ± 26 | 58 ± 3 | 0.46 ± 0.02 | 47 ± 2 |
% fresh | 85 ± 12 | 70 ± 3 | 90 ± 9 | 65 ± 4 | 65 ± 3 |
Mean | |||||
Fresh | 247 ± 52 | 1210 ± 90 | 64 ± 1 | 0.73 ± 0.01 | 71 ± 24 |
Cryopreserved | 184 ± 34 | 804 ± 52 | 50 ± 4 | 0.48 ± 0.01 | 44 ± 6 |
% fresh | 77 ± 7 | 67 ± 8 | 79 ± 7 | 66 ± 0.8 | 62 ± 11 |
Mean values are given ±S.E.M.; N.D., not determined.
↵1-a Metabolite production in pmol/min/slice. Peak areas of other metabolites (6β-OH, 19-OH, 14α-OH, 16α-OH, 16β-OH, and androstenodione) were measured and maintained at 62 to 114%, 54 to 71%, and 73 to 117% fresh slices for liver 1, 2, and 3, respectively. Total peak area of metabolites formed (including androstenodione) was 97, 59, and 94% fresh slices, respectively.
↵1-b Metabolite production in pmol/min/slice.
↵1-c Percentage of intact cells in slice cross-section.