Substrate | In Vitro Clbile | Predicted3-aClBin | In Vivo ClBin | IPRL ClBin |
---|---|---|---|---|
ml/min/kg | ||||
Rh123 | 8.0 ± 0.8 | 19.9 ± 5.83-b | 18.3 ± 1.83-c | 12.3 ± 1.23-d |
Digoxin | 4.9 ± 0.83 | 13.5 ± 5.83-b | 14.7 ± 3.73-e |
↵3-a Predicted ClBin(intrinsic biliary clearance) was calculated according to the linear relationship between intrinsic in vivo biliary clearance and the in vitro biliary clearance in SC rat hepatocytes for taurocholate, [d-pen2,5]enkephalin, methotrexate, salicylate, and inulin as established previously (in vivo ClBin = 2.04 × in vitro Clbile + 3.52; Liu et al., 1999b).
↵3-b Standard deviation on estimation of predicted values from linear relationship between in vitro and in vivo biliary clearance.
↵3-c Intrinsic biliary clearance of unchanged Rh123 in vivo in rats (Sweatman et al., 1990) calculated as described under Experimental Procedures.
↵3-d Intrinsic biliary clearance of unchanged Rh123 in rat IPRL (Jager et al., 1997) calculated as described under Experimental Procedures.
↵3-e Intrinsic biliary clearance of unchanged digoxin in rat IPRL (Hedman and Meijer, 1998) calculated as described under Experimental Procedures.