Biotransformation-related enzyme activities, intracellular GSH level, and γ-GT activity in freshly isolated rat type II pneumocytes and alveolar macrophages
| Type II Pneumocytes | Alveolar Macrophages | |
|---|---|---|
| EROD (pmol/mg/min) | Below detection limits1-a | Below detection limits1-a |
| PROD (pmol/mg/min) | 8.8 ± 0.8 (3) | Below detection limits1-a |
| BROD (pmol/mg/min) | 27.2 ± 1.5 (3) | 0.74 ± 0.201-160 (4) |
| NADPH-cytochrome c reductase (nmol/mg/min) | 42.9 ± 2.8 (5) | 28.0 ± 2.81-160 (5) |
| NADH-cytochrome c reductase (nmol/mg/min) | 75.5 ± 13.7 (5) | 31.7 ± 5.51-165 (4) |
| DT-diaphorase (nmol/mg/min) | 423 ± 119 (5) | 72.6 ± 50.21-160 (4) |
| GST (nmol/mg/min) | 104 ± 31 (6) | 29.0 ± 11.51-150 (5) |
| GSH (nmol/mg) | 17.4 ± 3.6 (5) | 18.8 ± 7.0 (6) |
| γ-GT (mU/mg) | 22.7 ± 4.8 (6) | Below detection limits1-b |
All enzyme activities were measured in sonicated cells and expressed per milligram of total cell protein. Data are the mean ± S.D.; the values in parentheses show n values.
↵1-150 p < 0.05,
↵1-160 p < 0.01,
↵1-165 p< 0.001 significant versus freshly isolated type II pneumocytes; unpaired Student's t test.
↵1-a <0.15 pmol of resorufin per min with the amount of cell protein used in the assay, i.e. 200 to 500 μg.
↵1-b <0.13 mU with the amount of cell protein used in the assay, i.e. 20 to 40 μg.