Specificity of Immunoblotting activity of monoclonal antibodies to expressed isoforms of the P450 2C subfamily
| mAb/P450 | 2C8 | 2C9*1 | 2C9*2 | 2C9*3 | 2C18 | 2C19 | 1A1, 1A2, 2A6, 2B6, 2D6, 2E1, 3A4, 3A5 |
|---|---|---|---|---|---|---|---|
| mAb 292-2-3 | −5-a | − | − | − | − | − | − |
| mAb 763-15-5 | − | − | − | − | − | − | − |
| mAb 763-15-20 | ++ | ++ | +++ | ++ | − | − | − |
| mAb 592-2-5 | − | ++ | +++ | ++ | +++ | − | − |
| mAb 327-8-3 | − | ++ | +++ | ++ | ++ | − | − |
| mAb 5-7-5 | − | ++ | +++ | ++ | + | ++ | − |
| mAb 1-68-11 | − | − | − | − | − | − | − |
↵5-a Binding intensity was rated as follows: −, no binding; +, weak binding; ++, strong binding; +++, very strong binding. Qualitative values are described underMaterials and Methods.