Reaction | Compound | Compound + GSH (Mono-Conjugate) | Compound + 2 GSH (Bi-Conjugate) | Hydroxylated Compound (HO-Catechol) | Hydroxylated Compound + GSH (Mono-Conjugate) |
---|---|---|---|---|---|
Compound + HRP/H2O2 | |||||
CA4-a | 181 (50%) | 486 (100%) | 791 (9%) | ||
CA4-b | 181 (45%) | 486 (100%) | 791 (15%) | 197 (11%) | |
CGA4-a | 355 (6%) | 660 (100%) | 965 (6%) | ||
CGA4-b | 355 (100%) | 660 (3%) | 371 (20%) | ||
DHCA4-a | 183 (100%) | 488 (33%) | 793 (80%) | 199 (13%) | 504 (7%) |
DHCA4-b | 183 (100%) | 488 (33%) | 793 (50%) | 199 (33%) | 504 (33%) |
Compound + Tyrosinase/O2 | |||||
CA4-a | 181 (40%) | 486 (20%) | 197 (100%) | ||
CA4-b | 181 (59%) | 486 (20%) | 197 (100%) | ||
CGA4-a | 355 (42%) | 660 (42%) | 371 (100%) | ||
CGA4-b | 355 (21%) | 660 (100%) | 371 (29%) | ||
DHCA4-a | 183 (57%) | 488 (15%) | 199 (100%) | ||
DHCA4-b | 183 (92%) | 488 (18%) | 199 (100%) |
Glutathione (4 mM) was added either before or after HRP (3 μM)/H2O2 (2 mM) addition to a CGA, CA, or DHCA (1 mM) solutions in Millipore filtered water. Tyrosinase (25 unit/ml) was also used instead of HRP/H2O2. Results are reported asm/z [M + 1]+ (relative intensity percentage).
↵4-a GSH was present at the beginning of the reaction.
↵4-b GSH was added 5 min after the reaction started.