Effect of an ionophore and metabolic inhibitors on the uptake of 0.5 μM YH18855
| Ionophore or Metabolic Inhibitors | Uptake | |
|---|---|---|
| Rate | Control | |
| pmol/cm2/40 s | % | |
| Control | 6.0 ± 0.6 | 100.0 |
| FCCP (10 μg/ml) | 4.3 ± 0.23-150 | 71.7 |
| 2,4-Dinitrophenol (1 mM) | 4.9 ± 0.63-150 | 82.4 |
| Sodium azide (10 mM) | 4.8 ± 0.23-150 | 79.5 |
After preincubation of the Caco-2 cell monolayer for 15 min with the indicated compounds on the apical side, the uptake of 0.5 μM YH1885 from the apical side was measured under the pH gradient conditions (6.5/7.4 for apical/basolateral pH). In these experiments, glucose was removed from the incubation medium in the apical and basolateral sides. Data represent mean ± S.D. of three experiments.
↵3-150 P <0.05 from Student's ttest.