Michaelis-Menten kinetics for different CYP marker reactions
| CYP | Reaction | Enzyme Kinetic Parameters | |||
|---|---|---|---|---|---|
| Human Liver Microsomes | Recombinant CYPS | ||||
| Km | Vmax | Km | Vmax | ||
| μM | nmol/min/mg protein | μM | nmol/min/nmol | ||
| 1A2 | Phenacetin | 43 | 2.5 | 107 | 7.8 |
| O-Deethylation | |||||
| 2C9 | Diclofenac | 1.5 | 1.7 | 8.3 | 15 |
| 4-Hydroxylation | |||||
| 2C19 | S-Mephenytoin | N.D. | N.D. | 54 | 2.14 |
| 4-Hydroxylation | |||||
| 2D6 | Bufuralol | 9.3 | 0.12 | 15 | 8 |
| 1′-Hydroxylation | |||||
| 3A4 | Testosterone | 71 | 6.8 | 55 | 8 |
| 6β-Hydroxylation | |||||
N.D., not determined.