Table 3

Kinetics of FNTZ biotransformation in cDNA-expressed enzymes

CYPDM FNTZ Formation3-OH FNTZ Formation
Vmax3-aKm3-bA3-cVmax/Km3-dVmax3-aKm3-bA3-cVmax/Km3-d
2A61.001920.50.520.13297.50.44
2B63-e 0.27100.72.68
2C90.3850.22.77.57
2C190.8359.613.93
3A41.54154.69.9615.7285.71.454.95

  • ↵3-a  Vmax in units of pmol min−1 (pmol CYP)−1.

  • ↵3-b  Km in units of μM.

  • ↵3-c  A, Hill coefficient.

  • ↵3-d  Vmax/Km, approximate intrinsic clearance, in units of nl min−1 (pmol CYP)−1.

  • ↵3-e  2B6, fit to eq. 3,z = 0.0002.