The effect of saccharic acid 1,4-lactone on UDP-glucuronosyl transferase activity in tissue microsomes
| Tissue | UDP-Glucuronosyl Transferase Activity | |||||
|---|---|---|---|---|---|---|
| Gemfibrozil | Valproic Acid | |||||
| −Saccharic Acid 1,4-Lactone | +Saccharic Acid 1,4-Lactone | Control | −Saccharic Acid 1,4-Lactone | +Saccharic Acid 1,4-Lactone | Control | |
| nmol/min/mg protein | % | nmol/min/mg protein | % | |||
| HLM | 0.87, 0.82 | 0.73, 0.66 | 82 | 0.97, 1.0 | 0.72, 0.94 | 84 |
| HKM | 0.51, 0.45 | 0.41, 0.42 | 86 | 1.3, 1.2 | 1.2, 1.1 | 92 |
| DLM | 4.8, 4.6 | 4.4, 4.2 | 91 | 2.3, 2.3 | 2.3, 2.4 | 100 |
HLM were produced by pooling microsomes prepared form six human livers, each one characterized as shown in Fig. 1. HKM were prepared form the kidney of a single human. DLM were prepared from the liver of a single beagle dog. Data represent mean from two individual expts. performed in duplicate.