Anti-CYP3A4 | 1,8-Cineole 2-Hydroxylation | |||
---|---|---|---|---|
HL-11 | HL-104 | |||
mg IgG/nmol P450 | nmol/min/nmol P450 | % | nmol/min/nmol P450 | % |
0.0 | 33.6 | (100) | 56.5 | (100) |
4.5 | 6.0 | (18) | 6.2 | (11) |
9.0 | 2.4 | (7) | 3.4 | (6) |
Liver microsomes (0.05 nmol of P450) were first incubated with indicated amounts of anti-CYP3A4 IgG for 5 min (at room temperature), and then 1,8-cineole and other components were added as described underMaterials and Methods. Parentheses indicate percentage of controls (in the absence of antibodies). Data are means of duplicate determinations.