Effects of PMSF and/or VC on [14C]NDMA demethylase activity (pmol of [14C]HCHO formed/mg of protein/min) in human lung microsomal incubations1-a
| Patient | Experiment | ||||
|---|---|---|---|---|---|
| +VC | +NADPH | +VC + NADPH | +PMSF + NADPH | +PMSF + VC +NADPH | |
| 1 | 82.36 ± 2.40 | 81.43 ± 2.57 | 51.80 ± 0.821-b | 80.86 ± 1.80 | 35.23 ± 2.061-b 1-c |
| 2 | 56.90 ± 1.59 | 56.57 ± 1.55 | 30.30 ± 0.621-b | 54.43 ± 3.31 | 17.03 ± 0.471-b 1-c |
| 3 | 80.10 ± 2.01 | 79.40 ± 0.70 | 43.27 ± 1.401-b | 78.97 ± 1.70 | 24.13 ± 2.951-b 1-c |
| 4 | 58.90 ± 1.94 | 60.17 ± 0.90 | 32.67 ± 1.261-b | 61.13 ± 1.96 | 17.37 ± 0.671-b 1-c |
| 5 | 72.13 ± 2.79 | 74.60 ± 2.02 | 40.53 ± 0.601-b | 73.03 ± 1.27 | 20.90 ± 1.611-b 1-c |
| 6 | 59.31 ± 2.91 | 64.60 ± 1.75 | 30.03 ± 2.931-b | 65.10 ± 1.42 | 14.27 ± 0.781-b 1-c |
| 7 | 36.91 ± 1.00 | 33.33 ± 0.45 | 10.90 ± 0.551-b | 32.17 ± 0.50 | 7.73 ± 0.211-b 1-c |
| 8 | 54.20 ± 3.51 | 57.57 ± 2.15 | 22.60 ± 0.621-b | 57.30 ± 0.98 | 15.38 ± 0.621-b 1-c |
| 9 | 62.05 ± 3.10 | 65.20 ± 2.85 | 28.03 ± 1.791-b | 64.73 ± 1.79 | 16.44 ± 1.491-b 1-c |
| 10 | 69.30 ± 4.40 | 68.40 ± 1.41 | 34.80 ± 0.561-b | 68.97 ± 1.38 | 16.83 ± 0.441-b 1-c |
↵1-a Incubations were conducted with VC and NADPH (VC + NADPH), with PMSF and NADPH (PMSF + NADPH), or with PMSF, VC, and NADPH (PMSF + VC + NADPH). Values are mean ± S.D. of triplicate determinations in simultaneous incubations from the same microsomal preparations of individual patients.
↵1-b p < 0.05 compared with levels in (+ VC), (+NADPH), and (+ PMSF + NADPH).
↵1-c p < 0.05 compared with levels in (+ VC + NADPH).