Primers and PCR conditions for PCR-RFLP
| Location3-a | Primers | Primer Sequences | PCR Conditions (35 Cycles) | Endonuclease |
|---|---|---|---|---|
| A13989G | Exon 5(S) | CACATTTTCTACAACCATGGAGACC | 45 s 94°C | BsmAI |
| Ile118Val | Exon 5(R) | AAATATGGACAGGGGTGGTCTAAG | 45 s 66°C | (mut) |
| (Exon 5) | 30 s 72°C | |||
| C15820G | Exon 7(S) | TGTTGCATGCATAGAGGAAGGATGG | 45 s 94°C | ClaI |
| Pro218Arg | Exon 7(R) | TACTGTCCCAAACACTGTCCC | 45 s 70°C | (mut) |
| (Exon 7) | 45 s 72°C | |||
| A17776insertion | Exon 9(S) | GAGCCATATTCTCAGAAGGGAGATCAAG | 45 s 94°C | HinfI |
| (Exon 9) | Exon 9(R) | GTTTGTACACAGCAAGACGATACACC | 45 s 69°C | (wt) |
| 30 s 72°C |