Table 3

Effect of centrifugal force on specific activities or contents: An example of ranking

HLMCentrifugation Force (g)
Activity or Content/mg of ProteinRank
9,00010,50012,0009,00010,50012,000
Positive Indicators
 NADPH cytochrome P450 reductase3-a 383.964.664.21.32.32.3
440.932.726.01.02.03.0
560.946.044.41.32.32.3
 Cytochrome P4503-b 30.3980.3980.5362.32.31.3
40.3570.2470.3571.72.71.7
50.3160.4810.2752.01.03.0
 NADH cytochrome b5 reductase3-a 30.2330.2200.2271.52.52.0
40.2110.2010.1771.02.03.0
50.1240.1270.1121.71.72.7
 Cytochromeb53-b 30.3720.3720.3451.71.72.7
40.3240.2970.3722.03.01.0
50.3310.2770.3451.72.71.7
 p-Nitrophenol hydroxylase3-a 31.461.052.102.03.01.0
41.310.901.181.03.02.0
52.052.050.681.71.72.7
 Tolbutamide hydroxylase3-a 30.4650.4300.2451.02.03.0
40.1720.1540.1631.03.02.0
50.2690.2540.2291.02.03.0
 DextromethorphanO-demethylation3-a 30.6230.6110.6432.02.51.5
40.2440.2350.2231.71.72.7
50.4930.5010.4331.71.72.7
 DextromethorphanN-demethylation3-a 30.4660.5200.5733.02.01.0
40.1660.1530.1381.02.03.0
50.1040.1000.1022.02.02.0
 UGT 1-naphthol3-a 316.05.710.01.03.02.0
47.911.18.93.01.02.0
59.15.84.91.02.03.0
 UGT morphine3-a 36.51.73.31.03.02.0
42.52.02.11.32.32.3
53.12.71.31.02.03.0
 p-Nitrophenolacetate hydrolysis3-a 30.3300.3690.3382.31.32.3
40.2550.2790.2312.01.03.0
50.0830.0890.0632.01.03.0
Negative Indicators
 Succinate dehydrogenase3-a 353.141.462.62.01.03.0
455.349.857.92.31.32.3
554.545.950.33.01.02.0
 GST CDNB3-a 30.3050.3250.4391.02.03.0
40.3430.2610.2233.02.01.0
50.1870.1930.1472.32.31.3
 Absorbance at 4203-b 30.0000.0000.3151.71.72.7
40.0560.1460.0681.03.02.0
50.2820.3720.0682.03.01.0
Mean rank1.72.12.2

  • GST CDNB, glutathione S-transferase 1-chloro-2,4-dinitrobenzene; UGT, UDP-glucuronosyltransferase.

  • ↵3-a  (nmol) (min)−1 (mg protein)−1.

  • ↵3-b  (nmol) (mg protein)−1.