Substrate (10 μM) | Sulfating Activities | ||||
---|---|---|---|---|---|
ST1A5 (Human) | ST1A3 (Human) | ST1A1 (Rat) | St1a4 (Mouse) | ST1A8 (Rabbit) | |
nmol/nmol/min [% of ST1A3 activity] | |||||
Tyramine | 4.34 ± 0.02 [6199] | 0.07 ± 0.01 [100] | 0.08 ± 0.04 [118] | 0.26 ± 0.02 [367] | 0.03 ± 0.01 [46] |
Norepinephrine | 3.98 ± 0.16 [7955] | 0.05 ± 0.00 [100] | 0.18 ± 0.01 [366] | 0.18 ± 0.00 [367] | 0.06 ± 0.00 [115] |
Normetanephrine | 3.33 ± 0.07 [2559] | 0.13 ± 0.00 [100] | 0.04 ± 0.01 [33] | 0.05 ± 0.01 [40] | 0.03 ± 0.00 [22] |
Dopamine | 3.07 ± 0.18 [1025] | 0.30 ± 0.01 [100] | 0.39 ± 0.02 [132] | 0.59 ± 0.07 [196] | 0.13 ± 0.01 [42] |
Epinephrine | 2.93 ± 0.05 [3254] | 0.09 ± 0.00 [100] | 0.10 ± 0.00 [112] | 0.12 ± 0.00 [130] | 0.03 ± 0.00 [28] |
HMPG | 0.69 ± 0.03 [95] | 0.72 ± 0.02 [100] | 1.08 ± 0.03 [150] | 1.58 ± 0.01 [219] | 2.65 ± 0.06 [368] |
DOPAC | 0.06 ± 0.00 [23] | 0.27 ± 0.01 [100] | 0.54 ± 0.01 [199] | 0.77 ± 0.01 [285] | 0.30 ± 0.00 [110] |
Assays were performed with 10 μM substrates at pH 7.4. After 20-min incubations, reaction mixtures were separated on thin layer plates. The radioactive spots were quantified by BAS1000. Each value represents mean ± S.D. (n = 3).
HMPG, 4-hydroxy-3-methoxyphenylglycol; DOAPC, 3,4-dihydroxyphenylacetic acid.