Table 1

HPLC condition for logD7.0 measurement of HMG-CoA reductase inhibitors

HMG-CoA Reductase InhibitorColumnMobile PhaseWavelength
Pravastatin (acid)Deverosil ODS-UG-51-a 80:20238 nm
Atorvastatin (acid)Deverosil ODS-UG-565:35238 nm
Cerivastatin (acid)Deverosil ODS-UG-560:40238 nm
Fluvastatin (acid)Deverosil ODS-UG-565:35238 nm
Atorvastatin lactoneCapcel pak C18 1-b 50:50237 nm
Cerivastatin lactoneCapcel pak C18 30:70237 nm
Fluvastatin lactoneInertsil ODS-21-c 65:35254 nm

Columns used were 150 × 4.6 mm. For mobile phase, 1 mM KH2PO4 (pH 7.0 NaOH): Acetonitrile was used in varying ratios as shown under Mobile Phase. The flow rate in all cases was 1 ml/min.

  • 1-a Deverosil: Nomura Chemical Co., Ltd. (Aichi, Japan).

  • 1-b  Capcel pak: Shiseido Co., Ltd. (Tokyo, Japan).

  • 1-c  Inertsil: GL Science Inc. (Tokyo, Japan).