Table 2

Sulfation of tracer [³H]E₁ in the cytosol fraction of zonal hepatocytes (n = 4) and zonal lysates (n = 4) in the presence of the cosubstrate PAPS (700 μM)

	Sulfation Intrinsic Clearance of Estrone	PP/PV Ratio
	μl/min/mg protein
Zonal Hepatocytes
PP	6.1 ± 2.2	0.23
PV	26.4 ± 9.5^2-150	0.23
Zonal Lysates
Periportal (LP)	8.3 ± 4.3	0.32
Perivenous (LV)	25.9 ± 6.8^2-160	0.32

↵2-150 Statistically significant from periportal (ANOVA,P < 0.05).
↵2-160 Statistically significant from periportal (pairedt test, P < 0.05).