Sample | Km (RG 12525) | Vmax | CLint |
---|---|---|---|
μM | nmol/min/mg | μl/min/mg | |
RPR-HL-10 | 16.6 | 4.38 | 264 |
RPR-HL-18 | 21.1 | 3.54 | 168 |
RPR-HL-20 | 19.9 | 5.94 | 298 |
Apparent Km and Vmax values were determined from linear regression analysis of Eadie-Hofstee plots using an integrated form of the Michaelis-Menten equation to minimize error in Km (Segel, 1976).
HL, human liver.