Table 1

Effect of different test agents on ABZ-SG formation by methemoglobin

ConditionABZ-SG Formation
% Control
Complete100
+1 mM NaCN12
+0.1 mM Ascorbic acid0
+50 mM DMPO8
+1 mM Thiourea0
+0.3 mM NaN3 4
+1 mM Histidine59
+10 μg/ml Catalase0
+50 mM Mannitol108
+2 μg/ml Superoxide dismutase195

The complete reaction contained 0.06 mM [3H]ABZ, 3 μM methemoglobin, 0.3 mM glutathione, and 0.05 mM H2O2. These conditions metabolized 1.6 nmol ofN-acetylbenzidine and produced 1.2 nmol of ABZ-SG. Values represent the average of at least duplicate determinations.