Table 1

Assays of individual P450-catalyzed metabolism of substrates used for inhibition kinetics

Substrate	Conc. range	P450	Product Formed	Internal Standard	Assay	Reference
	μM
Estrone	6.25–400	CYP1A2	2-OH-Estrone	BA t-5,6-diol	HPLC	Shou et al., 1997
Phenacetin	3.9–250	CYP1A2	Acetaminophen	Temazepam	LC-MS	See text
Ethoxyresorufin	0.05–50	CYP1A2	Resorufin	External standard	Fluorescence	See text
Diazepam	1–500	CYP2B6	Nordiazepam	BA t-5,6-D	HPLC	Yang et al., 1998
Paclitaxel	1.6–200	CYP2C8	6α-OH-Paclitaxel	Bacctin	HPLC	Rahman et al., 1994
Flurbiprofen	15.6–1000	CYP2C9	4′-OH-Flurbiprofen	BA t-5,6-D	HPLC	Tracy et al., 1995
Celecoxib	0.5–30	CYP2C9	Hydroxycelecoxib	Diclofenac	HPLC	Tang et al., 2000
		CYP2C9^*2
		CYP2C9^*3
(S)-Mephenytoin	1.56–150	CYP2C19	4′-OH-M	Nirvanol	HPLC	Goldstein et al., 1994
Dextromethorphan	3.0–2000	CYP2D6	Dextrorphan	BA t-5,6-diol	HPLC	See text
Chlorzoxazone	2–1000	CYP2E1	6-OH-C	BA t-5,6-diol	HPLC	Peter et al., 1990
Testosterone	6.26–1250	CYP3A4	6β-OH-T	6β-OH-Progesterone	HPLC	Hanioka et al., 1990
Progesterone	2.5–500	CYP3A4	6β-OH-P	Estradiol	HPLC	See text
Benzyloxyresorufin	2.5–50	CYP3A4	Resorufin	External standard	Fluorescence	See text

6-OH-C, 6-OH-chlorzoxazone; 6β-OH-T, 6β-OH-testosterone; 6β-OH-P, 6β-OH-progesterone; BA t-5,6-D, BAt-5,6-diol; LC-MS, liquid chromatography-mass spectrometry.