Cumene hydroperoxide-supported rates of phenytoin oxidations in liver microsomes from untreated and phenytoin-administered rats
| Rat Liver Microsomes | Product Formation | |
|---|---|---|
| 4′-HPPH | 3′,4′-Dihydrodiol | |
| pmol/min/nmol P450 | ||
| Untreated | 29 ± 4 | 310 ± 95 |
| Phenytoin-treated | 44 ± 54-160 | 890 ± 714-160 |
Phenytoin (100 μM) was incubated with rat liver microsomes at 37°C for 5 min in the presence of 100 μM cumene hydroperoxide. Data are mean ± S.D. for four rats.
↵4-160 Significantly different from untreated group (p< 0.01).