Table 2

Comparison of human P450 metabolism of PhIP

Enzyme4′-OH-PhIPN2-OH-PhIP
CYP1A1 V max 7.7  ± 0.516.1  ± 0.9
K m 8.2  ± 2.15.1  ± 1.2
V max/K m 0.943.2
CYP1A2 V max 1.5  ± 0.1589.9  ± 8.6
K m 42.7  ± 10.879.0  ± 16.9
V max/K m 0.041.14
CYP1B1 V max 0.3  ± 0.10.2  ± 0.1
K m 2.2  ± 0.44.5  ± 1.2
V max/K m 0.140.04

Vmax units: nmol min−1 nmol P450−1; Km units: μM.

  • Vmax/Km, intrinsic clearance (taken from Crofts et al., 1998).