Table 2

A sample set of 40 oligonucleotide probes for the G191A polymorphism taken from the first array design

Probe (1)	3′-5′ Sequence	L	Tm	% GC
191A_3′A	TTCTTTGGTACCACCCA	17	61.09	0.47
191A_3′A_NC	TGGGTGGGAACAAAGAA	17	61.09	0.47
191A_3′C	TTCTTTGGTCCCACCCA^2-a	17	63.50	0.53
191A_3′C_NC	TGGGTGGGACCAAAGAA^2-a	17	63.50	0.53
191A_3′G	TTCTTTGGTGCCACCCA	17	63.50	0.53
191A_3′G_NC	TGGGTGGGAGCAAAGAA	17	63.50	0.53
191A_3′T	TTCTTTGGTTCCACCCA	17	61.09	0.47
191A_3′T_NC	TGGGTGGGATCAAAGAA	17	61.09	0.47
191A_5′A	TTCTTTGATCCCACCCA	17	61.09	0.47
191A_5′A_NC	TGGGTGGAACCAAAGAA	17	61.09	0.47
191A_5′C	TTCTTTGCTCCCACCCA	17	63.50	0.53
191A_5′C_NC	TGGGTGGCACCAAAGAA	17	63.50	0.53
191A_5′G	TTCTTTGGTCCCACCCA^2-a	17	63.50	0.53
191A_5′G_NC	TGGGTGGGACCAAAGAA^2-a	17	63.50	0.53
191A_5′T	TTCTTTGTTCCCACCCA	17	61.09	0.47
191A_5′T_NC	TGGGTGGTACCAAAGAA	17	61.09	0.47
191G_3′A	TTCTTTGGCACCACCCA	17	63.50	0.53
191G_3′A_NC	TGGGTGGGGACAAAGAA	17	63.50	0.53
191G_3′C	TTCTTTGGCCCCACCCA^2-b	17	65.91	0.59
191G_3′C_NC	TGGGTGGGGCCAAAGAA^2-b	17	65.91	0.59
191G_3′G	TTCTTTGGCGCCACCCA	17	65.91	0.59
191G_3′G_NC	TGGGTGGGGGCAAAGAA	17	65.91	0.59
191G_3′T	TTCTTTGGCTCCACCCA	17	63.50	0.53
191G_3′T_NC	TGGGTGGGGTCAAAGAA	17	63.50	0.53
191G_5′A	TTCTTTGACCCCACCCA	17	63.50	0.53
191G_5′A_NC	TGGGTGGAGCCAAAGAA	17	63.50	0.53
191G_5′C	TTCTTTGCCCCCACCCA	17	65.91	0.59
191G_5′C_NC	TGGGTGGCGCCAAAGAA	17	65.91	0.59
191G_5′G	TTCTTTGGCCCCACCCA^2-b	17	65.91	0.59
191G_5′G_NC	TGGGTGGGGCCAAAGAA^2-b	17	65.91	0.59
191G_5′T	TTCTTTGTCCCCACCCA	17	63.50	0.53
191G_5′T_NC	TGGGTGGTGCCAAAGAA	17	63.50	0.53
191GA_A	TTCTTTGGACCCACCCA	17	63.50	0.53
191GA_A_NC	TGGGTGGGACCAAAGAA^2-a	17	63.50	0.53
191GA_C	TTCTTTGGCCCCACCCA^2-b	17	65.91	0.59
191GA_C_NC	TGGGTGGGCCCAAAGAA	17	65.91	0.59
191GA_G	TTCTTTGGGCCCACCCA	17	65.91	0.59
191GA_G_NC	TGGGTGGGGCCAAAGAA^2-b	17	65.91	0.59
191GA_T	TTCTTTGGTCCCACCCA^2-a	17	63.50	0.53
191GA_T_NC	TGGGTGGGTCCAAAGAA	17	63.50	0.53

Highlighted probe sequences are perfectly complementary to both strands of the two polymorphic forms of the NAT2 gene at the G191A polymorphic site. “C” and “NC” in the probe name designate the coding and noncoding strand sequences, respectively. Substituted nucleotide positions are in bold type.

Tm, temperature at which 50% of a DNA duplex denatures.
↵2-a A allele.
↵2-b G allele.