Kinetics of N-oxygenation for purified lung rFMO2-535 and expressed microsomal mFMO2-5352-a
| DMA N -Oxygenation2-b | (S) -Nicotine N′ -Oxygenation | |||||
|---|---|---|---|---|---|---|
| Km | Vmax | Vmax/ Km | Km | Vmax | Vmax/ Km | |
| μM | min−1 | ml · M−1 · min−1 | μM | min−1 | ml · M−1 · min−1 | |
| rFMO2-535 | 310 | 10.1 | 32.6 | 500 | 0.6 | 1.2 |
| mFMO2-5352-a | 670 | 38.2 | 57.0 | 1700 | 6.9 | 4.1 |
↵2-a Turnover for mFMO2-535 expressed in insect microsomes assumes a specific content of 1.7 nmol FMO · mg−1 of microsomal protein (mean from Table 1).
↵2-b The correlation coefficients for the lines used to estimate Km and Vmax were as follows: DMA N -oxygenation, r = 0.994 and 0.936 for rFMO2-535 and mFMO2-535, respectively; (S) -nicotine N -oxygenation, r =0.999 for rFMO2-535 and mFMO2-535.