Substrate | Rate at pH 8.5, pmol 3-OH-BaP formed/min/mg protein | |||
---|---|---|---|---|
Microsomes | Cytosol | |||
Proximal | Distal | Proximal | Distal | |
BaP-3-glucuronide | 2.0 ± 0.3 | 0.42 ± 0.21 | 0.07 ± 0.03 | 0.20 ± 0.09 |
BaP-3-sulfate | 0.44 ± 0.16 | 0.17 ± 0.08 | 0.87 ± 0.05 | 0.37 ± 0.26 |
Values shown are mean ± S.D., n = 4. The rates were obtained at pH 8.5, the pH of the buffer used in the in situ studies, in the presence of 10 μM substrate.