Table 4

Gradients of BaP-3-sulfate, BaP-3-glucuronide, and total 3-OH-BaP equivalents from mucosa to blood and mucosa to postinfusate following perfusion/incubation of catfish in situ intestinal preparations with micelles containing either 2 or 20 μM [3H]3-OH-BaP for 60 min

Treatment, Concentration of 3-OH-BaPMucosa to Blood Gradients4-aMucosa to Post-infusate Gradients
Total 3-OH-BaP Equivalents4-bBaP-3-SulfateBaP-3-GlucuronideTotal 3-OH-BaP EquivalentsBaP-3-SulfateBaP-3-Glucuronide
Control, 2 μM61.7  ± 11.536.4  ± 4.517,300  ± 7,9000.95  ± 0.133.6  ± 0.38.8  ± 3.0
BNF, 2 μM44.3  ± 7.337.9  ± 5.924,200  ± 8,6000.93  ± 0.107.0  ± 1.38.8  ± 2.3
Control, 20 μM78.5  ± 12.153.0  ± 7.01,944  ± 6791.76  ± 0.396.9  ± 1.214.7  ± 5.1
BNF, 20 μM64.3  ± 9.337.6  ± 5.618,600  ± 10,9001.32  ± 0.503.8  ± 0.67.5  ± 1.6

Catfish were either controls or treated with β-naphthoflavone (10 mg/kg diet) for 14 days.

  • ↵4-a  The gradients shown are the ratio of metabolite concentration in mucosa to that in blood or postinfusate. (For example, a mucosa to blood gradient of 10 represents a mucosa metabolite concentration 10 times that in blood). Gradient concentrations were calculated for each individual fish and presented as mean ± S.E. (n = 6 for 2 μM control and BNF;n = 4 for 20 μM control, and n = 5 for 20 μM BNF). Note that in the groups infused with 2 μM 3-OH-BaP, three control and five BNF-induced fish had no detectable BaP-3-glucuronide (concentration set at half the limit of detection for purposes of calculations). In the groups infused with 20 μM 3-OH-BaP, one BNF-treated fish had undetectable BaP-3-glucuronide. Mucosa to blood gradients were significantly steeper (p< 0.01) than mucosa to postinfusate gradients for the glucuronide and sulfate conjugates and total 3-OH-BaP molar equivalents.

  • ↵4-b  The total 3-OH-BaP equivalents were calculated from the total [3H]3-OH-BaP molar equivalents per gram of mucosa or per milliliter of blood or postinfusate.