Table 1

Glucuronidation of LA, 13-HODE, and 13-OXO by human liver microsomes and recombinant UGT2B7

Substrate	Enzyme Source	Enzymatic Activity
		nmol/mg·min
Linoleic Acid	HLM18	2.08 ± 0.28^1-a
	UGT2B7	1.19 ± 0.38^1-a
13-OXO	HLM18 and 2	2.26 ± 0.58^1-b
	UGT2B7	1.69 ± 0.29^1-b
13-HODE	HLM18 and 2
R_f = 0.31		1.91 ± 0.08^1-a
R_f = 0.45		0.80 ± 0.03^1-c
	UGT2B7
R_f = 0.31		0.98 ± 0.04^1-a
R_f= 0.45		0.46 ± 0.06^1-c

Enzymatic activity was determined with 100 μM substrate, 4 mM UDPGlcUA, and 50 μg of protein as described under Materials and Methods. Results are expressed as the mean ± S.D. for at least four determinations.

↵1-a Carboxyl-linked glucuronide.
↵1-b Glucuronide structure not determined.
↵1-c Hydroxyl-linked glucuronide.