Comparison of in vivo percutaneous absorption of [14C]DBP in haired male and female or hairless male Sprague-Dawley rats
| Haired Male Rats (n = 9) | Haired Female Rats (n = 4) | Hairless Male Rats (n = 6) | |
|---|---|---|---|
| Urine 0–72 h6-a | 38.1 ± 1.7 | 34.0 ± 5.2 | 57.0 ± 1.86-150 |
| Urine 0–4 h | 0.3 ± 0.1 | 0.4 ± 0.2 | 0.7 ± 0.16-150 |
| Urine 4–8 h | 1.4 ± 0.2 | 1.8 ± 0.5 | 3.6 ± 0.76-150 |
| Urine 8–24 h | 12.1 ± 0.7 | 10.3 ± 1.7 | 30.9 ± 2.76-150 |
| Urine 24–48 h | 13.7 ± 0.8 | 12.6 ± 3.1 | 18.0 ± 1.26-150 |
| Urine 48–72 h | 10.5 ± 0.8 | 8.9 ± 1.1 | 3.6 ± 0.36-150 |
| Feces 0–72 h6-a | 4.0 ± 0.5 | 1.3 ± 0.76-150 | 9.5 ± 0.66-150 |
| Carcass6-a | 13.1 ± 1.8 | 17.3 ± 2.7 | 1.8 ± 0.36-150 |
| Cage washing6-a | 5.8 ± 0.7 | 3.3 ± 0.76-150 | 2.6 ± 0.46-150 |
| Application site skin6-a | 0.8 ± 0.2 | 1.1 ± 0.2 | 0.2 ± 0.06-150 |
| Skin around the application site6-a | 7.3 ± 0.9 | 9.9 ± 3.4 | 0.5 ± 0.16-150 |
| Washing of the application site skin6-a | 21.2 ± 1.7 | 23.7 ± 1.5 | 25.0 ± 1.7 |
| Recovery6-a | 90.3 ± 1.0 | 90.8 ± 0.5 | 97.3 ± 0.66-150 |
| Percentage of the absorbed dose6-a | 61.1 ± 1.5 | 56.0 ± 4.4 | 70.9 ± 2.46-150 |
| Percentage of the penetrated dose6-a | 69.1 ± 1.5 | 67.0 ± 1.6 | 71.7 ± 2.4 |
| Plasma6-b | 0.0036 ± 0.0007 | 0.0034 ± 0.0006 | 0.001 ± 0.00016-150 |
| AUC0–72 hplasma6-c | 0.28 ± 0.02 | 0.37 ± 0.036-150 | 0.36 ± 0.036-150 |
| AUC0–infplasma6-c | 0.60 ± 0.08 | 0.61 ± 0.06 | 0.40 ± 0.036-150 |
| K el 6-d | 0.012 ± 0.02 | 0.019 ± 0.0046-150 | 0.050 ± 0.001 |
| Application site of skin6-e | 0.08 ± 0.02 | 0.11 ± 0.03 | 0.02 ± 0.0036-150 |
| Around application site of skin6-e | 0.27 ± 0.04 | 0.38 ± 0.15 | 0.02 ± 0.0046-150 |
| Weight of skin6-f | 0.17 ± 0.01 | 0.17 ± 0.01 | 0.22 ± 0.016-150 |
Twenty-four hours after a topical application of neat [14C]DBP (10 μl/cm2, 10 cm2) the application site skin was washed, and the animals were sacrificed 48 h later. Values are expressed as means ± S.E.M.
↵6-150 Significant difference from haired male rats group (p < 0.05).
↵6-a Percentage of applied dose.
↵6-b Percentage of the applied dose per ml of plasma.
↵6-c AUC plasma = % of the applied dose/ml × h.
↵6-d K el, elimination rate constant of 14C in plasma (1/h).
↵6-e Percentage of the applied dose per cm2.
↵6-f Gram per cm2 of skin.