Means of 14C recovery of LA and major metabolites in whole-body plasma following single oral administration of [14C]LA to male rats (30 mg/kg, n = 2) and male dogs (10 mg/kg, n = 3) and intravenous administration of [14C]LA to male dogs (10 mg/kg, n = 3)
| Species | Sampling Point (h) | 14C Recovery % of the Dose2-a | Relative Amounts % of Radioactivity2-b | Sum of Others | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| M3 | M6 | M8 | M10 | M11 | M12 | LA | ||||
| % | ||||||||||
| Rat p.o. | 1.00 | 2.9 | — | — | 27.0 | — | 29.7 | 10.9 | — | 32.4 |
| Rat p.o. | 3.00 | 1.7 | — | — | 29.1 | — | 34.4 | 6.2 | — | 30.3 |
| Dog p.o. | 1.00 | 4.8 | — | 13.9 | 19.3 | 19.7 | — | — | — | 47.1 |
| Dog p.o. | 2.00 | 6.9 | 7.6 | 3.0 | 20.5 | 24.7 | — | — | — | 44.2 |
| Dog p.o. | 4.00 | 3.9 | 20.7 | — | 18.1 | 13.8 | — | — | — | 47.4 |
| Dog i.v. | 0.08 | 17.4 | — | 23.5 | — | — | — | — | 23.3 | 53.2 |
| Dog i.v. | 0.25 | 12.2 | — | 44.6 | — | — | — | — | — | 55.4 |
| Dog i.v. | 0.50 | 11.2 | — | 16.9 | 10.5 | 9.0 | — | — | — | 63.6 |
| Dog i.v. | 1.00 | 10.2 | 4.3 | 12.8 | 12.6 | 15.4 | — | — | — | 54.9 |
| Dog i.v. | 2.00 | 7.8 | 6.7 | 4.8 | 20.7 | 23.1 | — | — | — | 44.7 |
| Dog i.v. | 4.00 | 4.3 | 8.0 | — | 21.9 | 19.8 | — | — | — | 50.3 |
↵2-a In the case of rats, the percentage of the dose was calculated on the basis of weight percentage of the total body weight using 4.02% for plasma (Spector, 1956; Waynforth, 1980), whereas for dogs the total body plasma was calculated from total blood volume (84.5 ml/kg), along with animal weights and plasma/blood ratios (Morton et al., 1993).
↵2-b Values represent relative peak areas expressed as percentage (i.e., 100% equals the sum of all peak areas in the respective radiochromatogram). The 14C recoveries obtained by SPE ranged between 40 and 70%.