Purification of P450 isoforms from small intestinal microsomes of monkeys
| Step | Total Protein | Total P450 | Specific Content | Recovery |
|---|---|---|---|---|
| mg | nmol | nmol/mg protein | % | |
| Microsomes | 2995 | 295 | 0.10 | 100 |
| Solubilized microsomes | 1486 | 288 | 0.19 | 98 |
| Aminooctyl Sepharose 4B | 208 | 183 | 0.88 | 62 |
| DEAE-5PW | ||||
| MI-11-a | 0.3 | 1.5 | 5.0 | 0.5 |
| MI-21-a | 3.2 | 10.5 | 3.3 | 3.6 |
| ES-502C | ||||
| MI-31-a | 1.8 | 12.3 | 6.8 | 4.2 |
↵1-a Detergent-removed fraction.