Chromatographic data of polar clozapine metabolites recovered from patient urine
| Substance | RF in Solvent | HPLC | Estimated Quantity | ||
|---|---|---|---|---|---|
| II | III | Eluent | RT | ||
| min | |||||
| 6-OH-CLZ-O-Sulf | 0.66 | 0.50 | 80:20 | 11 | + |
| 7-OH-CLZ-O-Gluc | 0.28 | 0.08 | 80:20 | 6.5 | + |
| 7-OH-CLZ-O-Sulf | 0.60 | 0.18 | 80:20 | 17 | + |
| 7-OH-DMCLZ-O-Sulf | 0.55 | 0.28 | 90:10 | 20 | +++ |
| 7-OH-CLZ-NO-O-Gluc | 0.19 | 0.13 | 80:20 | 11.5 | + |
| 7-OH-CLZ-NO-O-Sulf | 0.44 | 0.33 | 70:30 | 11 | ++ |
| 8-OH-CLZ-O-Gluc | 0.28 | 0.08 | 80:20 | 3.5 | + |
| 8-OH-DMCLZ-O-Gluc | 0.10 | 0.07 | 80:20 | 3.1 | ++ |
| 9-OH-DMCLZ-O-Sulf | 0.55 | 0.28 | 80:20 | 7 | + |
| CLZ-N +-Gluc | 0.37 | 0.40 | 70:30 | 25 | + |
| N-OH-DMCLZ-O-Gluc | 0.37 | 0.40 | 70:30 | 15 | + |
R F values refer to the first and second TLC separation. HPLC eluents were composed of 0.02 M ammonium acetate in 0.9 M acetic acid (pH 3.0) and methanol in the ratios shown in the column.
+, <0.2% of the dose; ++, 0.2 to 1%; +++, >1%.