Enzyme kinetic parameters for the glucuronidation of 1-HP in human liver microsomes (HLM) and by human recombinant UGTs
| Enzyme Source | Km | Vmax | Vmax/Km |
|---|---|---|---|
| μM | nmol/min/mg | μl/min/mg | |
| HLM 143-a | 8.6 ± 0.5 | 6.5 ± 0.09 | 756 |
| HLM 153-a | 8.6 ± 0.9 | 4.9 ± 0.34 | 570 |
| HLM 163-a | 7.4 ± 0.6 | 6.6 ± 0.24 | 892 |
| HLM3-b | 6.9 ± 0.6 | 5.8 ± 0.27 | 841 |
| UGT1A63-c | 45 ± 14 | 0.15 ± 0.02 | 3 |
| UGT1A73-d | 12 ± 0.4 | 0.15 ± 0.03 | 12 |
| UGT1A93-e | 1 ± 0.09 | 0.57 ± 0.03 | 568 |
↵3-a Microsomal protein (2 μg), 2.5 mM UDPGA, 0.1 to 300 μM 1-HP, 10 min.
↵3-b Microsomal protein (2 μg), 5 mM UDPGA, 0.1 to 300 μM 1-HP, 10 min.
↵3-c Cellular protein (1.4 μg), 2.5 mM UDPGA, 0.1 to 300 μM 1-HP, 10 min.
↵3-d Microsomal protein (15 μg), 2.5 mM UDPGA, 0.1 to 300 μM 1-HP, 10 min.
↵3-e Cellular protein (1.1 μg), 2.5 mM UDPGA, 0.1 to 300 μM 1-HP, 10 min.