Table 4

Effects of emodin and 3-methylcholanthrene on peroxide production, glutathione content, and drug-metabolizing enzyme activities in human lung adenocarcinoma CL5 cells

Assay	Control	Emodin	3-MC
Peroxide production (fluorescence unit)	397 ± 16	2,174 ± 284^4-a	354 ± 2
Glutathione content (nmol/mg of protein)	19.6 ± 0.2	39.3 ± 2.8^4-a	39.5 ± 0.7^4-a
Benzo[a]pyrene hydroxylation (pmol of OHBP/min/mg of protein)	0.1 ± 0.0	0.4 ± 0.1^4-a	3.9 ± 0.6^4-a
7-EthoxyresorufinO-deethylation (pmol of RF/min/mg of protein)	0.2 ± 0.1	2.6 ± 0.2^4-a	5.0 ± 0.1^4-a
7-EthoxycoumarinO-deethylation (pmol of HC/min/mg of protein)	0.1 ± 0.0	1.6 ± 0.4^4-a	6.8 ± 1.4^4-a

CL5 cells were treated with 100 μM emodin or 10 μM 3-MC for 24 h. Control cells were treated with DMSO only. Cell homogenate and S9 fractions were prepared for total glutathione content and drug-metabolizing enzyme activity determinations, as described underMaterials and Methods, respectively. In peroxide study, the cells were treated with 5 μM DCFH-DA for 2 h before analysis of fluorescence of DCFH using a flow cytometer. Each value represents mean ± S.E. for three experiments.

↵4-a Value significantly different from the respective control value, p < 0.05.