Metabolite | Treatment Group | Mouse | Rat |
---|---|---|---|
nmol/mg microsomal protein/60 min | |||
VCH-1,2-epoxide | control | 54.0 ± 8.01-b | 28.3 ± 2.8 |
phenobarbital | 318.6 ± 8.7 (5.9)1-c | 79.3 ± 7.1 (2.8) | |
VCH | 205.2 ± 36.5 (3.8) | 56.1 ± 5.3 (2.0) | |
VCH-7,8-epoxide | control | 36.6 ± 5.2 | 22.0 ± 5.3 |
phenobarbital | 75.7 ± 1.3 (2.1) | 35.0 ± 3.3 (1.6) | |
VCH | 75.0 ± 5.0 (2.0) | 39.6 ± 5.3 (1.8) | |
VCD | control | N.D. | N.D. |
phenobarbital | 7.6 ± 0.9 | N.D. | |
VCH | 6.3 ± 0.9 | N.D. |
N.D., not-detectable.
↵1-a n = 4 groups of microsomes were pooled from mice (4 mice/group, 16 mice total/treatment). Microsomes were prepared from individual rats (four rats per treatment).
↵1-b Values represent mean (±S.D.) nanomoles of epoxide formed per milligrams of protein after 60 min.
↵1-c Values in parenthesis represent significant (p < 0.05) fold-increase in epoxide metabolite formation over control microsomal incubations.