Insect Microsomes Expressing P450 and NADPH-P450 Reductase | Heat Inactivation of Microsomes | |
---|---|---|
+ | − | |
β-galactosidase activity (units/ml) | ||
Control | 230 | 230 ± 20 |
CYP1A1 | 200 | 220 ± 20 |
CYP1A2 | 220 | 210 ± 20 |
CYP1B1a (P220 lot 1)2-a | 210 | 890 ± 60 |
CYP1B1b (P220 lot 3)2-a | 190 | 790 ± 80 |
CYP1B1c (P220 lot 5)2-a | 210 | 890 ± 60 |
CYP2A6 | 200 | 200 ± 20 |
CYP2B6 | 230 | 210 ± 22 |
CYP2C8 | 230 | 230 ± 18 |
CYP2C9 | 240 | 270 ± 30 |
CYP2C18 | 240 | 230 ± 24 |
CYP2C19 | 240 | 250 ± 20 |
CYP2D6 | 240 | 230 ± 24 |
CYP2E1 | 210 | 250 ± 20 |
CYP3A4 | 220 | 220 ± 22 |
CYP3A5 | 220 | 230 ± 18 |
CYP3A7 | 210 | 220 ± 22 |
CYP4A11 | 210 | 200 ± 20 |
Insect microsomes (expressing different forms of human P450s together with human NADPH-P450 reductase) that were treated with or without heat inactivation (90°C for 2 min) were incubated with the bacterial suspension in the absence of an NADPH-generating system for 120 min, and the induction of umu gene expression was determined by measuring β-galactosidase activity. Data are mean of single determination (with heat inactivation) or means of duplicate determinations ± range (without heat inactivation).
↵2-a Lot number (GENTEST).